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Clustering RBH hits into files by reference gene
I have performed RBH for each of five transcriptomes against a reference genome, producing five separate output files. I figured, for ease, it was best...Last edited by mfort001; 06-20-2016, 10:54 AM. -
Appropriate Adapter Sequence fora trimming? RNAseq Transcriptome
For previous studies I have contracted out both Illumina sequencing and RNAseq analysis of transcriptomes. An updated reference genome was added to NCBI... -
RNA-Seq bacteria 2.9Mb
Hello,
What is the recommended number of reads for RNA-Seq (whole transcriptome) of bacteria with genome size of 2.9Mb? I saw about 10... -
Mapping to transcripts rather than to the genome in RNA Seq
I inherited an RNA-Seq project with the goal of looking at differential expression across different environmental conditions and species in protists (using... -
new at this and need some guidance
Hi all,
I am new at this so I hope I am posting in the right section.
I am building a tool that compares transcriptomes of the same species.... -
Cloud Computing for Assembly?
Is anyone working on this? I am interested in using cloud computing for a de novo Solexa assembly of mammal transcriptome. Looked at Amazon, but they... -
De Novo Assembly of a transcriptome
Hi all,
We are planning to perform an mRNA-seq run using the Illumina GAII platform. We are worried about assembling the transcriptome when we... -
Mapping transcriptomes by RNA-Seq
The beginning of the end for microarrays?
Jay Shendure
nature methods | July 2008 issue
Two complementary approaches, both using...
