I need help (or tips) for creating my own indexing-primers for Illumina seqeuncing (TrueSeq-compatible):
It seems to me that I could simply...
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Creating Custom dual index primers for Illumina Seqeuncing
Last edited by jov14; 04-24-2018, 08:33 AM. -
FeatureCounts highest counts for unstranded option
Dear All,
I am working on RNASeq data which is generated using Truseq straded HT kit.
I have done removing adapters and... -
TruSeq DNA Single Indexes Sets: How many samples on one lane
Hi
I'm new to Illumina sequencing and would like to run a few tests on a MiSeq platform using a TruSeq DNA Nano kit.
As... -
Pooling and sequencing NexteraXT and TrueSeq PCR free libraries on a single lane
Hi everyone,
I have a question regarding the pooling of NexteraXT and TrueSeq PCR free libraries onto a single Illumina HighSeq lane. I... -
Illumina HiSeq k-mers
Hi
This is about the Illumina Hiseq based on Trueseq library.
Prior to trimming, the fastqc showed some k-mers enrichment in the... -
miRNA precipitation prior to Illumina TruSeq Small RNA lib prep
Hi, I have isolated small RNA using the miRvana kit and would like to concentrate my product for input into the TrueSeq Small RNA library prep kit and... -
Illumina Truseq Exome 62Mb - variant metrics?
Hey Everyone,
I'm just curious what kind of variant metrics everyone obtains for the Truseq 62 Mb exome enrichment. I've only processed... -
Clip truseq adapter sequence from fastq
Here's my public history https://main.g2.bx.psu.edu/u/davidkim/h/ngsworkshop
I am trying to learn NGS using the public data www.ncbi.n... -
default RNA-Seq library prep
What with all the developments in the RNA-Seq library prep field our lab is contemplating moving away from the Illumina Tru-Seq RNA kits and using the... -
TruSeq RNA-How many samples to pool?
Hey,
I would like to pool only 4 or 5 TruSeq RNA libraries and i am wondering which indexes to used? Previously, Illumina provided some... -
library concentrations at various levels (RNA seq)
Hi,
I am interested in some library preparation details of RNA seq.
Lets assume you deal with GA or HISeq.
I am just... -
Mate-Pair Illumina library problems
Hi, I am about to start a resequencing project on the HiSeq 1000 searching for rearrangements in a bacterial genome.
This is the 1st time I am...
