Hello all,
I am a trainee scientist about to begin my research project which involves the setup of RNAseq in a clinical laboratory. ...
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mRNA library prep kit selection
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Custom TruSeq-equivalent WGS library preps, for high number of smal genomes
Does anyone have experience with custom high troughput "TruSeq-equivalent WGS library preps"?
Normal TruSeq library prep costs... -
AmpliSeq vs TruSeq
Now that Illumina sells AmpliSeq, I am trying understanding the difference between AmpliSeq for Illumina and TruSeq Targeted panels. From what I can tell...Last edited by seqsuave; 03-14-2019, 12:44 PM. -
Combining TruSeq and Nextera XT Indices
Hello people,
we usually prepare and run the samples on our MiSeq using the 26 6nt-long i7 TruSeq DNA Indices (formerly TruSeq LT, I think).... -
Truseq Rapid Exome TArget Bed file's Gene Names?
İs there a way to get gene names of the Truseq Rapid exome Target Bed file whihc only includes target coordinates? -
Adapters for Nextera Mate Pair Library Prep Kit
Dear,
I have a question about Nextera Mate Pair Library Prep Kit (https://www.illumina.com/products/by...its/library-pr... -
Multiplexing BluePippin size selection
First post, but a longtime lurker!
Currently doing Illumina library preps for MiSeq sequencing, and even though we have mostly used TruSeq... -
RNA-seq of long RNAs (paired-end, stranded) -- fragmentation time
I'm using the TruSeq Stranded Total RNA library preparation kit to generate libraries for 2x150 bp paired-end sequencing. Illumina's fragmentation protocol... -
Overcoming PCR Primer Bias with MiSeq?
Hi, I am a grad student new to MiSeq and HTS in general. I'm trying to figure out which sequencing kit/method to use for my project, and I'm getting some... -
SPRI dilution in Illumina Library Prep
Hello everyone,
I'm actually a newbie in this field, and this forum has helped me a lot with some issues.
I've been reading a few Illumina... -
Can I remove index adapters after assembly?
I assembled a reference transcriptome de novo using Trinity with 2x300bp Illumina MiSeq PE reads. I used libraries from 4 individuals that were multiplexed... -
script for checking barcode compatibility
Hi everyone,
a colleague of mine wrote a script that checks the compatibility of barcodes used in different sample prep kits for Illumina... -
Truseq Custom Amplicon kit overhang sequences
I have been searching for for the overhang sequence on the i7 and i5 indices of the Truseq Custom amplicon Index kit, but couldn't find them..
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Concentrating method for TruSeq lib prep in microRNA sequencing
Hi all,
We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).
We use column-based... -
HiSeq4000 small RNA libraries (vs 2500)
Hi All,
I'm having some issues running a custom small RNA sequencing protocol on the HiSeq4000, when the same library works perfectly fine...
