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  • candicechu
    Junior Member
    • Nov 2015
    • 3
    #1

    Concentrating method for TruSeq lib prep in microRNA sequencing

    Hi all,

    We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

    We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples.

    I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method?

    Any suggestions are appreciated. Thanks!
    Tags: concentrate, microrna, microrna sequencing, truseq
    • nucacidhunter
      Jafar Jabbari
      • Jan 2013
      • 1250
      #2
      SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.

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