Hello everyone,
I’m working with Pool-Seq data (allele frequency data from pooled samples) and I’d like to perform PCA and other multivariate...
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Filtering rare variants and variance-stabilizing transformations for Pool-Seq PCA
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Variance in cDNA amplification
Hello,
I recently generated cDNA from 100 T-cells (from the same source) using the Smart-Seq2 protocol from Sandberg et al. After the pre-amplification...
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Variance modelling in DESeq2
Hi there,
My apologies if this is a stupid question. I've just moved into RNA-seq analysis, and am trying to figure out some issues....
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Cufflinks performs poorly with biological variance
Hi,
I may just not have found the correct set of options, but Cufflinks performs very poorly with my data to the extent that I don't think...
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Variance Estimation
I have been using DESeq to analyze gene expression from SAGE samples. To decide how to compare samples we have been using ECDF (empirical cumulative data...