Hi guys,
I'm doing some ChIP-seq for k4me3 and k27me3 using the GAII (illumina) and although I see a clear difference in my control PCR (comparing on and off genes) I can not see any difference after the sequencing! it seems that the reads are in all the genome with out a peak in the promoter of off genes (in the case of K27me3).
does anyone have an idea of what can be the reason?
I'm doing some ChIP-seq for k4me3 and k27me3 using the GAII (illumina) and although I see a clear difference in my control PCR (comparing on and off genes) I can not see any difference after the sequencing! it seems that the reads are in all the genome with out a peak in the promoter of off genes (in the case of K27me3).
does anyone have an idea of what can be the reason?
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