All I can say is (some) DNA oligos must run not true to the markers on the RNA chips. It just goes against everything I know about PCR. Sometimes you have to believe the data, but sometimes the data is misleading. Someone needs to mass spec them. We have a mass spec but not the PPC oligos. Weird stuff!!!!!
And if the oligos are so long, why does Illumina say to use a 60˚ C anneal temp for the PCR? Is there something that I am missing completely?
And if the oligos are so long, why does Illumina say to use a 60˚ C anneal temp for the PCR? Is there something that I am missing completely?
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