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  • ChIP-seq fastq.gz files

    Hi,

    I recently completed my first ChIP-seq experiment.
    I received fast.gz files from the sequencing company.
    Obviously I now need to analyze this data.
    How can I convert this data to a file format compatible with the USCS genome browser such as BAM or BED?

    Any suggestions for analysis other than needing a good bioinformatician?

  • #2
    Nope - you just answered your own question

    You need someone to unzip and map the reads (fastq) to a reference genome (hg19?) and give you the aligned reads (bam) - and format a bedfile for showing the bamfile on ucsc.

    If this makes no sense at all or if you are unfamiliar with linux software such as samtools, bwa and bowtie - then call the bioinformatics department. What you are asking is a simple and trivial task.

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