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Have you seen such base distribution plot in BS-seq?
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If by BS-seq you mean Bisulfite Sequencing I have to say NO. Conversion has failed or all Cs were methylated! -
I concur with nucacidhunter and add "Yikes, that's bad!"Comment
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If the experiment is 2x125bp paired-end sequencing and both were concatenated into this one plot and the sequencing is non-directional and you used some kind of target enrichment technique or random primed pull-down then there might be a chance that there is something to be had from the data. But only you would know your library that well...
If this is not the case, I would like to agree with the above posters.
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This plot indicates a very high level of adapter dimer fragments in your library. Filter these out with your favorite trimming/filtering tool and then repeat the base composition plot to see what your valid library fragments look like.Comment
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
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