Hi.
I'm reading this paper (doi:10.1038/nature13415) and i'm trying to interpret some data from that plot
Please correct me if i'm wrong.
After a Hi-C experiment they found some sites (boundaries sites) where one place of the chromosome interacts with another (and possibly creates loops).
Below the boundaries sites there is a list of some regulation proteins like CTCF and histones like H3K27me3.
So if there is such a question :
"Is the CTCF insulator protein demarcating boundaries between 3D or chromatin domains?"
could the answer be :
"CTCF protein isn't demarcating boundaries between neither 3D (Hi-C) nor chromatin domains because it seems that it doesn't bind only at the boundaries sites but in a much more sites within the chromosome"
?
I'm reading this paper (doi:10.1038/nature13415) and i'm trying to interpret some data from that plot
Please correct me if i'm wrong.
After a Hi-C experiment they found some sites (boundaries sites) where one place of the chromosome interacts with another (and possibly creates loops).
Below the boundaries sites there is a list of some regulation proteins like CTCF and histones like H3K27me3.
So if there is such a question :
"Is the CTCF insulator protein demarcating boundaries between 3D or chromatin domains?"
could the answer be :
"CTCF protein isn't demarcating boundaries between neither 3D (Hi-C) nor chromatin domains because it seems that it doesn't bind only at the boundaries sites but in a much more sites within the chromosome"
?
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