I am trying to make ChIP work, and my no-antibody control group (chromatin-protein + beads) enriched as much as my positive control (anti-RNA Pol II). Post-IP I washed each ChIP reaction in three different wash buffers a total of six washes. Is this a finesse step? I pipetted beads up and down to mix but not too terribly vigorously.
I was also told that blocking of the beads would not be necessary but maybe it is. Has anyone else found that helpful?
I was also told that blocking of the beads would not be necessary but maybe it is. Has anyone else found that helpful?
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