Anybody have any experience with protein affinity tags for ChIP in human cells. I've tried FLAG-M2 and it gives a lot of background. I also tried the STrEP II-tag and it also gives a lot of background. I'm expressing my protein from a weak promoter so background is more of an issue then using something like a CMV promoter.
Is there an affinity tag that works really well for ChIP?
Is there an affinity tag that works really well for ChIP?
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