HI everyone:
I use bowtie to map reads to genome on Galaxy using recommend option. and results like below (just one read show here):
HWUSI-EAS455_0034_FC630CNAAXX:4:1:1045:9169#0/1 16 chr10 10711690
255 40M * 0 0 GGATGTTGGAGTGCCCAGTGTACTCCATTTCAAAATACTA
HHHHHHDGHGHHHHGHHHHHHFGDGDGHHFHHHHHHHGGG XA:i:0 MD:Z:40 NM:i:0
could you point out which is indicate strand of my reads mapped to? I known 16 on the 2nd column indicates - and 4 is nomatch and do you know 0 indicates what? (0 is highly prevail in my data)
By the way there are always 0,4,16 in my data, Is it normal?
I use bowtie to map reads to genome on Galaxy using recommend option. and results like below (just one read show here):
HWUSI-EAS455_0034_FC630CNAAXX:4:1:1045:9169#0/1 16 chr10 10711690
255 40M * 0 0 GGATGTTGGAGTGCCCAGTGTACTCCATTTCAAAATACTA
HHHHHHDGHGHHHHGHHHHHHFGDGDGHHFHHHHHHHGGG XA:i:0 MD:Z:40 NM:i:0
could you point out which is indicate strand of my reads mapped to? I known 16 on the 2nd column indicates - and 4 is nomatch and do you know 0 indicates what? (0 is highly prevail in my data)
By the way there are always 0,4,16 in my data, Is it normal?
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