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  • visivas
    replied
    We found similar distribution of coverage for targeted resequencing experiment with %GC compared to whole genome. We had peak coverage in regions that had between 30%-70% GC content.

    Leave a comment:


  • Seq84
    started a topic Coverage assessment

    Coverage assessment

    Hi,

    We are making a target resequencing experiment in 6 differents regions. During coverage assessment with DepthOfCoverage walker in GATK we noticed that regions rich in GC (high %GC) were characterize by lower coverage values compare to ones in low-normal %GC regions.

    How the % in GC can affect coverage in a target resequencing experiment?

    Thanks in advance

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