Does anyone have any advice on denoising Illumina reads (specifically 16s reads from a HiSeq)? I've had a look in the literature and most of the denoising algorithms available seem to be aimed at 454 sequencing (and not really applicable to Illumina since they work on flowgram files). The only real possibility seems to be Rosen et al (2012). I would be very interested to hear about anyone's experience using this or any other approach for denoising Illumina data.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...