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Does anyone have any advice on denoising Illumina reads (specifically 16s reads from a HiSeq)? I've had a look in the literature and most of the denoising algorithms available seem to be aimed at 454 sequencing (and not really applicable to Illumina since they work on flowgram files). The only real possibility seems to be Rosen et al (2012). I would be very interested to hear about anyone's experience using this or any other approach for denoising Illumina data.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM