I'm currently running metagenomics pipeline through galaxy, and at the taxonomic mapping step I use ncbi blast+ wrappers to blast against a locally installed nt database. But ncbi nt database contains many data which are not totally relevant to metagenomics , so I want to locally install a more curated database like Silva or greengenes. But is it possible to do a megablast using blast+ wrappers against the silva or greengenes database? I have no idea of how to proceed. Any feedback or alternative suggestions are much appreciated.
Thanks a lot.
Thanks a lot.
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