Hi all!
I am doing a 16S amplicon analysis on soil samples with 5 different treatments.
I have 9 replicates per sample.
I got from illumina base space a file containing all the reads for all different taxonomic levels. Right now I am working on the class one.
I normalized the reads to the total number of reads. now I would like to verify the variability within my treatment between the reps, and obtain a single data set to work with per traetment.
Could you suggest me how is the best way to proceed with the analysis testing the variability, for istance, and then combine the sequences coming from the replicates into a unique sample sequence data set?
Could you suggest me the best method to analyze the variability between my replicates?
Do you have ideas?
Thank you very much!
I am doing a 16S amplicon analysis on soil samples with 5 different treatments.
I have 9 replicates per sample.
I got from illumina base space a file containing all the reads for all different taxonomic levels. Right now I am working on the class one.
I normalized the reads to the total number of reads. now I would like to verify the variability within my treatment between the reps, and obtain a single data set to work with per traetment.
Could you suggest me how is the best way to proceed with the analysis testing the variability, for istance, and then combine the sequences coming from the replicates into a unique sample sequence data set?
Could you suggest me the best method to analyze the variability between my replicates?
Do you have ideas?
Thank you very much!
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