Hi Friends
I know that mitochondrial sequences cannot be removed using any existing kits (Ribozero/Ribominus). How many sequences can be mitochondrial in any Illumina sequencing results? I sequenced few clones in my library using Sanger sequencing, most of them turn out to be mitochondrial and a couple of them rest but not rRNA. Do I need to optimize my cycle number? Any suggestion as to how should I try to optimize cycles?
Deepak
I know that mitochondrial sequences cannot be removed using any existing kits (Ribozero/Ribominus). How many sequences can be mitochondrial in any Illumina sequencing results? I sequenced few clones in my library using Sanger sequencing, most of them turn out to be mitochondrial and a couple of them rest but not rRNA. Do I need to optimize my cycle number? Any suggestion as to how should I try to optimize cycles?
Deepak
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