Hi,
when i am executing tophat like this:
./tophat -r 430 /mnt/FILE/zv9genome/zvgenome ../ex1/t_1 ../ex1/t_2
i got this exception:
[Sat Feb 4 18:00:11 2012] Beginning TopHat run (v1.4.0)
-----------------------------------------------
[Sat Feb 4 18:00:11 2012] Preparing output location ./tophat_out/
[Sat Feb 4 18:00:11 2012] Checking for Bowtie index files
[Sat Feb 4 18:00:11 2012] Checking for reference FASTA file
[Sat Feb 4 18:00:11 2012] Checking for Bowtie
Bowtie version: 0.12.7.0
[Sat Feb 4 18:00:11 2012] Checking for Samtools
Samtools Version: 0.1.18
[Sat Feb 4 18:00:11 2012] Generating SAM header for /mnt/FILE/zv9genome/zvgenome
format: fastq
quality scale: phred33 (default)
[Sat Feb 4 18:00:24 2012] Preparing reads
left reads: min. length=101, count=5992
right reads: min. length=101, count=5967
[Sat Feb 4 18:00:24 2012] Mapping left_kept_reads against zvgenome with Bowtie
gzip: stdout: Broken pipe
[Sat Feb 4 18:00:27 2012] Processing bowtie hits
Warning: junction database is empty!
[Sat Feb 4 18:02:17 2012] Processing bowtie hits
[FAILED]
Error executing: /mnt/FILE/tophat-1.4.0.Linux_x86_64/bam_merge ./tophat_out/tmp/left_kept_reads.candidates_and_unspl.bam ./tophat_out/tmp/left_kept_reads.candidates.bam ./tophat_out/tmp/left_kept_reads.unspl.bam
is the error result of the gzip broken pipe or from the junction database is empty?
Do anyone know what is the gzip exception?
Best,
Pap
when i am executing tophat like this:
./tophat -r 430 /mnt/FILE/zv9genome/zvgenome ../ex1/t_1 ../ex1/t_2
i got this exception:
[Sat Feb 4 18:00:11 2012] Beginning TopHat run (v1.4.0)
-----------------------------------------------
[Sat Feb 4 18:00:11 2012] Preparing output location ./tophat_out/
[Sat Feb 4 18:00:11 2012] Checking for Bowtie index files
[Sat Feb 4 18:00:11 2012] Checking for reference FASTA file
[Sat Feb 4 18:00:11 2012] Checking for Bowtie
Bowtie version: 0.12.7.0
[Sat Feb 4 18:00:11 2012] Checking for Samtools
Samtools Version: 0.1.18
[Sat Feb 4 18:00:11 2012] Generating SAM header for /mnt/FILE/zv9genome/zvgenome
format: fastq
quality scale: phred33 (default)
[Sat Feb 4 18:00:24 2012] Preparing reads
left reads: min. length=101, count=5992
right reads: min. length=101, count=5967
[Sat Feb 4 18:00:24 2012] Mapping left_kept_reads against zvgenome with Bowtie
gzip: stdout: Broken pipe
[Sat Feb 4 18:00:27 2012] Processing bowtie hits
Warning: junction database is empty!
[Sat Feb 4 18:02:17 2012] Processing bowtie hits
[FAILED]
Error executing: /mnt/FILE/tophat-1.4.0.Linux_x86_64/bam_merge ./tophat_out/tmp/left_kept_reads.candidates_and_unspl.bam ./tophat_out/tmp/left_kept_reads.candidates.bam ./tophat_out/tmp/left_kept_reads.unspl.bam
is the error result of the gzip broken pipe or from the junction database is empty?
Do anyone know what is the gzip exception?
Best,
Pap