Maybe if you were a bit more specific, you would get an answer.
"RPM" means "reads per million", i.e., you count how many reads map to your gene, divide by the total number of aligned reads and multiply by one million. So, I guess, (assuming that you know how to carry out a division) that you want to know how to count how many reads map onto a gene. My htseq-count script is one option, BEDtools or easyRnaSeq are others.
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM
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