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  • Simon Anders
    replied
    Maybe if you were a bit more specific, you would get an answer.

    "RPM" means "reads per million", i.e., you count how many reads map to your gene, divide by the total number of aligned reads and multiply by one million. So, I guess, (assuming that you know how to carry out a division) that you want to know how to count how many reads map onto a gene. My htseq-count script is one option, BEDtools or easyRnaSeq are others.

    Leave a comment:


  • swapnil2188
    replied
    Any one please help me with this.

    How should I calculate RPM values for a gene list ?

    Leave a comment:


  • swapnil2188
    started a topic How to Calculate RPM value for gene

    How to Calculate RPM value for gene

    How can I calculate RPM value for a gene to validate my results ?

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