Hi,
After mapping RNA-Seq reads with Tophat, I see reads mapped to non-exon regions.
My question is: will cufflinks take these reads when calculating gene/transcript expression level??????????
I hope not. Otherwise, the whole thing is not right.
Another question is; does anybody know where these "non-exon" reads come from????
Looking forward to your answers.
Thanks in advance.
After mapping RNA-Seq reads with Tophat, I see reads mapped to non-exon regions.
My question is: will cufflinks take these reads when calculating gene/transcript expression level??????????
I hope not. Otherwise, the whole thing is not right.
Another question is; does anybody know where these "non-exon" reads come from????
Looking forward to your answers.
Thanks in advance.
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