Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • MapSplice error

    hello,
    I am running MapSplice and I am able to get the output files, but there is empty in my best_junction.bed file.

    Furthermore, I got some error messenger form MapSplice:

    [Thu Jun 28 04:07:56 2012] Beginning Mapsplice run (v1.15.2)
    -----------------------------------------------
    bin directory: [/home/biongs158/Mapsplice_1.15.2/bin/]
    [Thu Jun 28 04:07:56 2012] Checking for chromosomes files or directory
    [Thu Jun 28 04:07:56 2012] Checking for chromosomes files or directory passed
    [Thu Jun 28 04:07:56 2012] Checking for Bowtie index files
    [Thu Jun 28 04:07:56 2012] check reads format
    [Thu Jun 28 04:08:43 2012] merge paired end reads remove short
    [Thu Jun 28 04:15:14 2012] Mapping reads against SRS000047_chr11_no_n with Bowtie
    [Thu Jun 28 04:20:06 2012] Converting bowtie mapped to SAM format
    [Thu Jun 28 04:20:52 2012] divide reads
    [Thu Jun 28 04:36:48 2012] Mapping reads against SRS000047_chr11_no_n with Bowtie
    [Thu Jun 28 04:50:55 2012] sort segmentbwt
    [Thu Jun 28 05:00:54 2012] reads all chromo sizes
    [Thu Jun 28 05:00:56 2012] mapsplice_search
    [Thu Jun 28 05:14:51 2012] Aligning spliced reads
    [Thu Jun 28 05:28:32 2012] mapsplice_report
    [Thu Jun 28 05:35:56 2012] remove index of sam
    [Thu Jun 28 05:35:59 2012] sort segmentbwt
    [Thu Jun 28 05:36:01 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:36:03 2012] sort by name
    [Thu Jun 28 05:36:33 2012] Remove duplication of sam
    [Thu Jun 28 05:37:09 2012] Filtering junctions
    [Thu Jun 28 05:37:09 2012] Remove duplication of sam
    [Thu Jun 28 05:37:12 2012] Filter Reads By Canon Noncanon
    [Thu Jun 28 05:37:12 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:13 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:14 2012] filter junction by min mis and min lpq
    [Thu Jun 28 05:37:14 2012] filter junc by ROC argu noncanonical
    [Thu Jun 28 05:37:14 2012] filter junction by min mis and min lpq
    [Thu Jun 28 05:37:14 2012] convert junction file to bed format
    [Thu Jun 28 05:37:15 2012] Filter Sam By junction
    [Thu Jun 28 05:37:16 2012] Merging all sam files
    [Thu Jun 28 05:37:20 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:26 2012] convert junction file to bed format
    [Thu Jun 28 05:37:26 2012] sort segmentbwt
    [Thu Jun 28 05:37:34 2012] Add tags to sam file
    [Thu Jun 28 05:47:31 2012] combine to final sam

    [Thu Jun 28 05:49:16 2012] Finishing Mapsplice run (time used: 1:41:20.265268)

    Does anyone have these error?
    Hope you can help me,thanks a lot!
    Last edited by sean760305; 06-28-2012, 01:31 AM.

  • #2
    Please email the author, I think he will be glad to help you.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      Yesterday, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 07:17 AM
    0 responses
    11 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-02-2024, 08:06 AM
    0 responses
    19 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-30-2024, 12:17 PM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-29-2024, 10:49 AM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Working...
    X