low input for RNA preparation - SEQanswers

You are currently viewing the SEQanswers forums as a guest, which limits your access. Click here to register now, and join the discussion

X

Seqwil

Junior Member

Join Date: Aug 2009

Posts: 2
- Share
- Tweet
#1

low input for RNA preparation

08-31-2009, 06:49 AM

Hello,

does anyone have experience using lower amount of starting material (total RNA) for the Illumina mRNA sequencing preparation (sep 2008)?
Did anyone tried lower then 1 ug?
Tags: None

Previous template Next

From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data

by SEQadmin2

Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...
- Channel: Articles
06-02-2026, 10:05 AM
Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends

by SEQadmin2

With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.

Introduction

Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
- Channel: Articles
05-22-2026, 06:42 AM

	Topics		Statistics	Last Post
	Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2 Started by SEQadmin2, Today, 06:09 AM		0 responses 11 views 0 reactions	Last Post by SEQadmin2 Today, 06:09 AM
	Sequencing the Two-Toed Sloth Genome Reveals Jumping Genes Tied to Its Extreme Metabolism by SEQadmin2 Started by SEQadmin2, 06-09-2026, 11:58 AM		0 responses 33 views 0 reactions	Last Post by SEQadmin2 06-09-2026, 11:58 AM
	A New Method Makes Hantavirus Genome Analysis Faster and More Accessible by SEQadmin2 Started by SEQadmin2, 06-05-2026, 10:09 AM		0 responses 38 views 0 reactions	Last Post by SEQadmin2 06-05-2026, 10:09 AM
	A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2 Started by SEQadmin2, 06-04-2026, 08:59 AM		0 responses 43 views 0 reactions	Last Post by SEQadmin2 06-04-2026, 08:59 AM

Working...