Hi, if anyone has experience on analyzing RNA-Seq data which come ScriptSep V2 libraries and Illumina sequencing?
I try to use tophat and set --library-type fr-secondstrand, however the -r/--mate-inner-dist is vaired based on ScriptSep library preparation.
other tools can be used?
I try to use tophat and set --library-type fr-secondstrand, however the -r/--mate-inner-dist is vaired based on ScriptSep library preparation.
other tools can be used?