Dear All,
I haven't analyzed fly transcriptome data. We are thinking of using r5.48 reference genome and annotation table. I found that there are many gene sets in one GFF file. Should I just extract one gene set from the file? If I use the antire table with all the gene sets, will that affect the FPKM calculation in Cufflinks? Thanks for all the help.
I haven't analyzed fly transcriptome data. We are thinking of using r5.48 reference genome and annotation table. I found that there are many gene sets in one GFF file. Should I just extract one gene set from the file? If I use the antire table with all the gene sets, will that affect the FPKM calculation in Cufflinks? Thanks for all the help.