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miR sequencing: miR and miR*, sense and antisense

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SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
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  • miR sequencing: miR and miR*, sense and antisense

    Hi:
    I was wondering what people are doing with their miRNA data to quantitate
    miR and miR* from their sequence reads (SOLID in my case).Especially novel miR*.
    (I wish they change miR* nomenclature to more sensible 3p-5p one)
    Is anybody aware of any computational approaches to automate miR vs miR*
    quantitation? Also I was wondering how people are addressing sense - antisense
    mapping issues related to ds regions in pre-miRs?
    Thanks

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