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  • which platform is better for de novo RNA-seq?

    Hi everyone, I am the newman in this field, and i want to explore the de novo RNA-seq, so which platform is better? thanks a million.

  • #2
    It depends on how much sequencing you need to answer the question you are asking. Some platforms have quirks, e.g. Illumina has problems with low complexity samples, 454 and Ion torrent has problems with miscalling homopolymers. Could you be more specific about what the sequencing is for?

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