Could anyone give me any advice on what proportion of DE genes would need to be validated using qPCR in an RNA Seq study with +8 biological replicates and if its common to just choose those above a certain fold change or would this bias results.
Are there high-throughput ways to do this kind of qPCR for a large group of genes?
Thanks in advance for any help!
Are there high-throughput ways to do this kind of qPCR for a large group of genes?
Thanks in advance for any help!
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