I tried to find the junctions for my fastq reads (single end reads, 42 bps). The segment_juncs provided me with 50lakhs possible junctions when trying to find in the whole genome. When I tried to align the same reads with a single chromosome as reference, I still got 5000000 possible junctions. Please explain why does it always give 5000000 outputs? Is there any possible way to reduce the no.of outputs?
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
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