Ribosome profiling vs. TRAP
I'm interested in doing ribosome profiling on a small bacterial genome. The "standard" protocols all call for isolating ribosomes with either sucrose cushions or (more recently with the Epicentre protocol) S-400 size exclusion. There seems to be an entirely different group doing ribosome isolation using affinity tags on ribosomal proteins, and they call their protocol TRAP. Both seem to get to the same place, but the affinity approaches surely seem easier and more amenable to automation. Does anyone know of why there is a reluctance to use the affinity techniques as an approach to ribosome profiling?
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
