Hello,
I am new to this so please bear with me if my question is too vague.
I have some small RNA-Seq (miRNA) sequencing data that I have aligned and mapped and have raw counts for, and I have a two-part question:
1) Does anyone have any literature that describes the "minimum relevant" count that a certain miRNA would need to be expressed at to possess its silencing activity? Obviously, this is relevant to the system/experiment, so any general literature on the subject would be great. (Google was not my friend today)
2) What is the best normalization technique for a small sample size (n=150) of miRNA small RNA-Seq experiment?
Thank you in advance.
I am new to this so please bear with me if my question is too vague.
I have some small RNA-Seq (miRNA) sequencing data that I have aligned and mapped and have raw counts for, and I have a two-part question:
1) Does anyone have any literature that describes the "minimum relevant" count that a certain miRNA would need to be expressed at to possess its silencing activity? Obviously, this is relevant to the system/experiment, so any general literature on the subject would be great. (Google was not my friend today)
2) What is the best normalization technique for a small sample size (n=150) of miRNA small RNA-Seq experiment?
Thank you in advance.
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