Dear All..
As a newbie in transcriptome analysis, I would like to ask a question about doing whole transcriptome assembly using trinity.
Is it possible for us to get two different transcripts when we assemble our reads by either concatenating the reads or listed the reads (using comma separation as Trinity manual says)?. I am just not sure with the results that I got, It seems that I got different transcripts (can tell this from its size which is different) using these two different method in preparing my reads for the assembly using Trinity. Any thought what went wrong ?
Cheers
Didi
As a newbie in transcriptome analysis, I would like to ask a question about doing whole transcriptome assembly using trinity.
Is it possible for us to get two different transcripts when we assemble our reads by either concatenating the reads or listed the reads (using comma separation as Trinity manual says)?. I am just not sure with the results that I got, It seems that I got different transcripts (can tell this from its size which is different) using these two different method in preparing my reads for the assembly using Trinity. Any thought what went wrong ?
Cheers
Didi
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