Hi members,
My intention is to get the promoter sequence of a a specific gene from paired end illumina RNASeq reads.
Now I aligned those sequences to the reference chromosome using Tophat and got the BAM file. At this point, I dont know how to accurately retrieve the promoter sequence of that gene from BAM file.
So, If anyone can suggest me the best method,it would be very helpful for me.
Thank you in advance...
anikng,
ROK
My intention is to get the promoter sequence of a a specific gene from paired end illumina RNASeq reads.
Now I aligned those sequences to the reference chromosome using Tophat and got the BAM file. At this point, I dont know how to accurately retrieve the promoter sequence of that gene from BAM file.
So, If anyone can suggest me the best method,it would be very helpful for me.
Thank you in advance...
anikng,
ROK
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