Hi all,
I want to do analysis of differentially expressed genes and splice-variants.
I have Illumina HiSeq2000 101bp paired end reads mapped to GRCh38, release 78, but I am uncertain how many mapped reads are 'enough' for a sample to be included, such that I in good conscious can say, that I use 'high quality' data?
1 million? 10 million? mapped reads/sample
Cheers,
Leon
I want to do analysis of differentially expressed genes and splice-variants.
I have Illumina HiSeq2000 101bp paired end reads mapped to GRCh38, release 78, but I am uncertain how many mapped reads are 'enough' for a sample to be included, such that I in good conscious can say, that I use 'high quality' data?
1 million? 10 million? mapped reads/sample
Cheers,
Leon
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