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Hi,
I am having some issues with RNA quality. This is the first time that my lab is doing RNA isolation, and we want to explore E.coli transcriptional response in the late stationary phase. I harvest the cells 10 hrs after entry in the stationary phase..
I use Qiagen RNEasy kit for RNA isolation, and have the data on total RNA QC using Agilent Bioanalyser. The RIN values for all samples are between 5-6,
I believe that this could be due to the fact that I am harvesting the bacteria in late stationary phase, which may already have some significant RNA degradation, and not due to the procedure used for RNA isolation.
Is the quality of the RNA good enough for RNA seq? Would you proceed with your samples?
Thank you in advance!
I am having some issues with RNA quality. This is the first time that my lab is doing RNA isolation, and we want to explore E.coli transcriptional response in the late stationary phase. I harvest the cells 10 hrs after entry in the stationary phase..
I use Qiagen RNEasy kit for RNA isolation, and have the data on total RNA QC using Agilent Bioanalyser. The RIN values for all samples are between 5-6,
I believe that this could be due to the fact that I am harvesting the bacteria in late stationary phase, which may already have some significant RNA degradation, and not due to the procedure used for RNA isolation.
Is the quality of the RNA good enough for RNA seq? Would you proceed with your samples?
Thank you in advance!