How many treatments can I have in a line RNA sequencing using MiSeq?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Depends on level of coverage you want. For reference, a MiSeq run will generate 25 M+ paired end reads of up to 300 bp each. You can use that yield to calculate theoretical coverage you will get. You can mix tens (or even hundreds) of properly barcodes samples. There is no limit there.
-
This depends on three questions:
Which type of RNA are you going to sequence?
Are you going to prepare the samples?
How much coverage do you want for your experiment?
The first question relies on the prep kit you need, if it is going to seq smRNA or mRNA, there are specialized kits.
The second question is if you have in mind to prep your libraries, then you only have the size indexing. The truseq RNA has a dual index system, which you may index up to 96 samples at the same time.
But... if you are going to aquire an external service, this technical prep is not up to you.
The third question relies on the biological meaning of your experimental design. what are you going to do? variant calling? exon sequencing? differential expression? splicing? Do you have target genes in mind? de novo transcriptomic profiling? low-expression transcripts? regulational rna? siRNA? lncRNA?
Comment
-
The experiment aims to analyze the expression of genes in a plant pathogen interaction. if possible I would also get small RNAs . however it is not a priority. to obtain expression profiles of messenger is enough. thanks for your reply . Another question is , if I get libraries with 6 good coverage . finally I will request an external service.
Comment
Latest Articles
Collapse
-
by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
Channel: Articles
05-06-2024, 07:48 AM -
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:57 AM
|
0 responses
12 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:57 AM
|
||
Started by seqadmin, 05-06-2024, 07:17 AM
|
0 responses
16 views
0 likes
|
Last Post
by seqadmin
05-06-2024, 07:17 AM
|
||
Started by seqadmin, 05-02-2024, 08:06 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
05-02-2024, 08:06 AM
|
||
Started by seqadmin, 04-30-2024, 12:17 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-30-2024, 12:17 PM
|
Comment