how many replicates are required for discovery of novel isoform using RNA-seq

[rajesh1989]

Hello everyone,

can I use RNA-seq data from 3 tissues each having 3,3,2 biological replicates and NO technical replicates to identify novel transcripts using cufflinks RABT based assembly?

if not then how many replicates do i need? Thank You.

[GenoMax]

For reference cross-posted: https://www.biostars.org/p/200725/

If is ok to cross-post here.