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  • jbrabec
    Junior Member
    • Mar 2017
    • 1

    #1

    SAM Header is present Error

    Hello Everyone!

    I am new to SEQanswers so not quite sure how to phrase my question but here goes:

    I am trying to run the command

    rsem-calculate-expression --bowtie2 --paired-end ~/Striata/cKO02/cKO02_R1.fastq ~/Striata/cKO02/cKO02_R2.fastq ~/rsem_reference/rsem_references ~/rsem_outputs/cKO02_counted

    And I get an error that reads
    [samopen] SAM header is present: 63759 sequences
    The program then stops at that point. Is there something wrong with my reference files that would be giving this error?

    Thanks in advance for your help. Let me know if you need more information!

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