Hi,
I recently did my first ScriptSeq mRNA libraries.
I am now analyzing the data and I see huge amounts of antisense reads mapping to the genes. I already did strand specific mRNAseq with a different method and there I did not see that much antisense.
It would be nice to know if somebody also experienced this problem and if there is a explanation for this.
Thank you
I recently did my first ScriptSeq mRNA libraries.
I am now analyzing the data and I see huge amounts of antisense reads mapping to the genes. I already did strand specific mRNAseq with a different method and there I did not see that much antisense.
It would be nice to know if somebody also experienced this problem and if there is a explanation for this.
Thank you
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