Hii all,
I am new to Bioinformatics field, i have never worked on any kind of statistical data analysis using tophat or bowtie or cufflink, so please excuse me for my naiveté. My querry is as follows:
I have two samples to compare one is a control and other is a knock-in. I did their RNA extraction -> cDNA prep -> Library prep -> Sequencing on Illumina Hi seq -> tophat -> bowtie -> cufflink -> Cuffcompare -> Cuffdiff.
Now after these many analysis i have so many output file (.gtf, .tracking, . refmap, .expr and so on) and i am so much confused as how and what to look into them.
Can anybody please tell me of any tutorial available where is can have an idea of how to further analyze my data in a step by step manner and find out significant differences between both of my samples.
Thanks in advance.
I am new to Bioinformatics field, i have never worked on any kind of statistical data analysis using tophat or bowtie or cufflink, so please excuse me for my naiveté. My querry is as follows:
I have two samples to compare one is a control and other is a knock-in. I did their RNA extraction -> cDNA prep -> Library prep -> Sequencing on Illumina Hi seq -> tophat -> bowtie -> cufflink -> Cuffcompare -> Cuffdiff.
Now after these many analysis i have so many output file (.gtf, .tracking, . refmap, .expr and so on) and i am so much confused as how and what to look into them.
Can anybody please tell me of any tutorial available where is can have an idea of how to further analyze my data in a step by step manner and find out significant differences between both of my samples.
Thanks in advance.
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