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I was uploading my RNA-seq assembly in TSA and I am not sure how to proceed. 743 sequences from my assembly got flagged with Code(VECTOR_MATCH) UniVec vector: gnl|uv|NGB00150.1:1-46 Ambion FirstChoice RLM-RACE 3' RACE adapter. I find it weird because I thought I already removed adapters prior to assembly using trimmomatic + fastqc. I have searched for the sequence of the adapter. What I want to know is how do I safely trim out the adapter sequence. I mean what if the vector is at the middle portion of the sequence, is it still acceptable to just remove the chunk and merge the two segments together? I could not find any sources to answer this at the moment that's why I am here.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM