Dear all,
I am struggling to get this command right, and getting errors all the time.
First Error:
$ STAR --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate
EXITING because fatal ERROR:not enough memory for BAM sorting.
SOLUTION: re-rrun STAR with at least --limitBAMsortRAM 123762737
I included the option in my command and this is what I got... and then
Second Error:
$ STAR --limitBAMsortRAM 123762737 --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate
EXITING because of FATAL ERROR: failed reading from temporary file: xxx_STARtmp//BAMsort/0/43
Can somebody help me out whit this? I have literally lost hope
Thank you in advance
I am struggling to get this command right, and getting errors all the time.
First Error:
$ STAR --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate
EXITING because fatal ERROR:not enough memory for BAM sorting.
SOLUTION: re-rrun STAR with at least --limitBAMsortRAM 123762737
I included the option in my command and this is what I got... and then
Second Error:
$ STAR --limitBAMsortRAM 123762737 --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate
EXITING because of FATAL ERROR: failed reading from temporary file: xxx_STARtmp//BAMsort/0/43
Can somebody help me out whit this? I have literally lost hope
Thank you in advance