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Dear all
We are studying a 1500bp gene. Cufflinks on RNA-seq from 2 different biological groups predicts 3 splice forms: one full-length one and two shortened :
Maq mapping of reads confirms higher expression at the 5' and 3' ends. This supports Cufflink's predictions.
first biological group :
second biological group :
However, no reads indicate polyadenylation of the the 3' end of first short splice form.
Thus we wonder if the 2 short splice forms are artifacts.
Any ideas what may be going on?
hugo
We are studying a 1500bp gene. Cufflinks on RNA-seq from 2 different biological groups predicts 3 splice forms: one full-length one and two shortened :
Maq mapping of reads confirms higher expression at the 5' and 3' ends. This supports Cufflink's predictions.
first biological group :
second biological group :
However, no reads indicate polyadenylation of the the 3' end of first short splice form.
Thus we wonder if the 2 short splice forms are artifacts.
Any ideas what may be going on?
hugo