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Hi everybody, we are trying to start a project that includes RNAseq. As this it is our first attempt we have plenty of unsolved questions and we would appreciate your help.
We are doing a project to analyze gene expression differences in two cell types. We are purifying RNA using Zymo research columns and after this we want to do a polyT selection prior RNAseq. Now we are thinking to add a DNase treatment in between to eliminate the possible remaining genomic DNA but we are concerned that this step is going to affect our RNA integrity. It is very important to add the DNase step? There are some protocol to know if we really have genomic DNA in our sample? Genomic DNA contamination could affect seriously our gene expression analysis?
Thank you very much in advance
We are doing a project to analyze gene expression differences in two cell types. We are purifying RNA using Zymo research columns and after this we want to do a polyT selection prior RNAseq. Now we are thinking to add a DNase treatment in between to eliminate the possible remaining genomic DNA but we are concerned that this step is going to affect our RNA integrity. It is very important to add the DNase step? There are some protocol to know if we really have genomic DNA in our sample? Genomic DNA contamination could affect seriously our gene expression analysis?
Thank you very much in advance