Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • lucatape
    Junior Member
    • Apr 2011
    • 1

    Cuffdiff Data Analysis

    Hi Everybody,

    my name is Luca and I work in NY at Albert Einstein Medical College as Postdoc.
    Part of my project will be to analyze the results coming from a RNAseq experiment on myeloid cells.

    The allignment data are good after TopHat and Cufflinks. The bioinformatic facility gave me a huge file containing the Cuffdiff statistics.
    this file contains all the genes with their KPFM.

    How should i prodeed now?

    I would like to have a list of genes stat differentially modulated, the total number of transcripts encoded, the number of unannotated genes..

    Anyone can help me please?

    Luca

Latest Articles

Collapse

  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
54 views
0 reactions
Last Post SEQadmin2  
Working...