I am trying to assemble human RNA-seq data using velvet. I also want to do a reference based assembly. Should i map the reads to the human genome or transcriptome? plz advice.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...