Hello every one....
My name is Ashis Sinha. I am from New Delhi (India). My work revolves around miRNAs and requires sequencing on the Illumina hiseq 2000 platform.
Its nice to meet everyone....
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
if anyone is familiar with the truseq cluster generation protocol, i would like some information on how much (in pico moles) should i load to have efficient cluster generation, illumina has a feature to discard if there is an overgeneration of clusters...
the protocol says upto 20 picomoles....but i think its too much.
Anything will be of help
My name is Ashis Sinha. I am from New Delhi (India). My work revolves around miRNAs and requires sequencing on the Illumina hiseq 2000 platform.
Its nice to meet everyone....
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
![Smile](https://www.seqanswers.com/core/images/smilies/smile.png)
if anyone is familiar with the truseq cluster generation protocol, i would like some information on how much (in pico moles) should i load to have efficient cluster generation, illumina has a feature to discard if there is an overgeneration of clusters...
the protocol says upto 20 picomoles....but i think its too much.
Anything will be of help
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