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  • #1

    ATACseq libray optimization

    Hi guys,
    I'm new to ATACseq and I just finished a library prep of 50K nuclei following the OMNI-ATAC protocol. I performed PCR amplification after Tagmentation (5 recommend cycles, plus 4 more cycles after qPCR). However, when I run Agilent (D5000) following amplification, I don't see a nice laddering as expected. I have attached my Agilent result.
    Should I do a bead clean up to remove those fragments beyond 1500bp? If so, what ration?
    Thank you for any help you can provide
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