It seems unlikely that LNA bases would be used - to my knowledge, LNA in the template inhibits most polymerases.
I assume the phosphorothioate linkage helps predominantly preventing 3’ to 5’ exonuclease activity of the ligase - reducing adapter dimers.
We too, had good experiences with the Bioo adapters though.
I assume the phosphorothioate linkage helps predominantly preventing 3’ to 5’ exonuclease activity of the ligase - reducing adapter dimers.
We too, had good experiences with the Bioo adapters though.
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